Gene description for Fam49b
Gene name family with sequence similarity 49, member B
Gene symbol Fam49b
Other names/aliases RGD1307697
Species Rattus norvegicus
 Database cross references - Fam49b
ExoCarta ExoCarta_299909
Vesiclepedia VP_299909
Entrez Gene 299909
 Fam49b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Fam49b
Molecular Function
    MHC class Ib protein binding, via antigen binding groove GO:0023030 ISO
    small GTPase binding GO:0031267 ISO
Biological Process
    positive regulation of T cell mediated cytotoxicity GO:0001916 ISO
    regulation of cell migration GO:0030334 ISO
    negative regulation of actin filament polymerization GO:0030837 ISO
    positive regulation of type II interferon production GO:0032729 ISO
    positive regulation of T cell activation GO:0050870 IBA
    positive regulation of T cell activation GO:0050870 ISO
    regulation of chemotaxis GO:0050920 ISO
    negative regulation of small GTPase mediated signal transduction GO:0051058 ISO
    cellular response to molecule of bacterial origin GO:0071219 ISO
    regulation of mitochondrial fission GO:0090140 ISO
    regulation of establishment of cell polarity GO:2000114 ISO
    positive regulation of memory T cell activation GO:2000568 ISO
Subcellular Localization
    mitochondrion GO:0005739 ISO
    cilium GO:0005929 ISO
    membrane GO:0016020 IEA
 Experiment description of studies that identified Fam49b in sEVs
1
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Fam49b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Fam49b is involved
No pathways found





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