ExoCarta: Gene summary

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Gene description for Exosc4
Gene name	exosome component 4
Gene symbol	Exosc4
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Exosc4
ExoCarta	ExoCarta_300045
Entrez Gene	300045
Exosc4 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Exosc4

Molecular Function
	exoribonuclease activity	GO:0004532	ISO
	AU-rich element binding	GO:0017091	ISO
Biological Process
	nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5'	GO:0034427	IBA
	polyadenylation-dependent snoRNA 3'-end processing	GO:0071051	IBA
	nuclear-transcribed mRNA catabolic process	GO:0000956	ISO
	nuclear mRNA surveillance	GO:0071028	ISO
	defense response to virus	GO:0051607	ISO
	U4 snRNA 3'-end processing	GO:0034475	IBA
	maturation of 5.8S rRNA	GO:0000460	ISO
	DNA deamination	GO:0045006	ISO
	positive regulation of cell growth	GO:0030307	ISO
	histone mRNA catabolic process	GO:0071044	ISO
	rRNA 3'-end processing	GO:0031125	IBA
	rRNA catabolic process	GO:0016075	IBA
Subcellular Localization
	cytoplasmic exosome (RNase complex)	GO:0000177	IBA
	cytoplasm	GO:0005737	ISO
	nucleus	GO:0005634	ISO
	nucleolus	GO:0005730	IBA
	exosome (RNase complex)	GO:0000178	ISO
	transcriptionally active chromatin	GO:0035327	ISO
	nuclear exosome (RNase complex)	GO:0000176	IBA

Experiment description of studies that identified Exosc4 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting