Gene description for Rbx1
Gene name ring-box 1, E3 ubiquitin protein ligase
Gene symbol Rbx1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rbx1
ExoCarta ExoCarta_300084
Entrez Gene 300084
 Rbx1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Rbx1
Molecular Function
    cullin family protein binding GO:0097602 ISO
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase activity GO:0061630 ISO
    ubiquitin-protein transferase activity GO:0004842 ISO
    protein complex binding GO:0032403 IPI
    NEDD8 transferase activity GO:0019788 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    ubiquitin-ubiquitin ligase activity GO:0034450 ISO
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    protein neddylation GO:0045116 ISO
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    protein catabolic process GO:0030163 ISO
    protein monoubiquitination GO:0006513 ISO
    protein ubiquitination GO:0016567 ISO
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 ISO
Subcellular Localization
    Cul2-RING ubiquitin ligase complex GO:0031462 ISO
    Cul3-RING ubiquitin ligase complex GO:0031463 ISO
    VCB complex GO:0030891 IDA
    Cul5-RING ubiquitin ligase complex GO:0031466 ISO
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IBA
    nuclear SCF ubiquitin ligase complex GO:0043224 IBA
    Cul7-RING ubiquitin ligase complex GO:0031467 ISO
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 ISO
    Cul4B-RING E3 ubiquitin ligase complex GO:0031465 ISO
    SCF ubiquitin ligase complex GO:0019005 ISO
    cullin-RING ubiquitin ligase complex GO:0031461 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Rbx1 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rbx1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rbx1 is involved
No pathways found





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