Gene description for SLC40A1
Gene name solute carrier family 40 (iron-regulated transporter), member 1
Gene symbol SLC40A1
Other names/aliases FPN1
HFE4
IREG1
MST079
MSTP079
MTP1
SLC11A3
Species Homo sapiens
 Database cross references - SLC40A1
ExoCarta ExoCarta_30061
Entrez Gene 30061
HGNC 10909
MIM 604653
UniProt Q9NP59  
 SLC40A1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for SLC40A1
Molecular Function
    protein binding GO:0005515 IPI
    iron ion transmembrane transporter activity GO:0005381 IMP
Biological Process
    anatomical structure morphogenesis GO:0009653 TAS
    iron ion transmembrane transport GO:0034755 IMP
    regulation of transcription from RNA polymerase II promoter in response to iron GO:0034395 IEA
    spleen trabecula formation GO:0060345 IEA
    transmembrane transport GO:0055085 TAS
    cellular iron ion homeostasis GO:0006879 TAS
    multicellular organismal iron ion homeostasis GO:0060586 IEA
    endothelium development GO:0003158 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    lymphocyte homeostasis GO:0002260 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
Subcellular Localization
    cytoplasm GO:0005737 TAS
    integral component of plasma membrane GO:0005887 IC
    integral component of membrane GO:0016021 TAS
    synaptic vesicle GO:0008021 IEA
    multivesicular body GO:0005771 IEA
    basolateral plasma membrane GO:0016323 IDA
    intracellular GO:0005622 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified SLC40A1 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for SLC40A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SLC40A1 is involved
PathwayEvidenceSource
Iron uptake and transport TAS Reactome
Metal ion SLC transporters TAS Reactome





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