Gene description for Tmem30a
Gene name transmembrane protein 30A
Gene symbol Tmem30a
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Tmem30a
ExoCarta ExoCarta_300857
Vesiclepedia VP_300857
Entrez Gene 300857
UniProt Q6AY41  
 Tmem30a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tmem30a
Molecular Function
    structural molecule activity GO:0005198 IEA
    structural molecule activity GO:0005198 ISO
    aminophospholipid flippase activity GO:0015247 IEA
    aminophospholipid flippase activity GO:0015247 ISO
Biological Process
    xenobiotic transmembrane transport GO:0006855 IEA
    xenobiotic transmembrane transport GO:0006855 ISO
    lipid transport GO:0006869 IEA
    positive regulation of neuron projection development GO:0010976 IEA
    positive regulation of neuron projection development GO:0010976 ISO
    aminophospholipid transport GO:0015917 ISO
    protein localization to endosome GO:0036010 IEA
    protein localization to endosome GO:0036010 ISO
    phospholipid translocation GO:0045332 IBA
    phospholipid translocation GO:0045332 ISO
    phospholipid translocation GO:0045332 ISO
    positive regulation of phospholipid translocation GO:0061092 IEA
    positive regulation of phospholipid translocation GO:0061092 ISO
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 IEA
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 ISO
    aminophospholipid translocation GO:0140331 IEA
Subcellular Localization
    photoreceptor outer segment GO:0001750 IEA
    photoreceptor inner segment GO:0001917 IEA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 ISO
    apical plasma membrane GO:0016324 IEA
    transport vesicle membrane GO:0030658 IEA
    early endosome membrane GO:0031901 IEA
    early endosome membrane GO:0031901 ISO
    late endosome membrane GO:0031902 IEA
    late endosome membrane GO:0031902 ISO
    phospholipid-translocating ATPase complex GO:1990531 IEA
    phospholipid-translocating ATPase complex GO:1990531 ISO
    phospholipid-translocating ATPase complex GO:1990531 ISS
 Experiment description of studies that identified Tmem30a in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 99
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tmem30a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tmem30a is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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