ExoCarta: Gene summary

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Gene description for Uba5
Gene name	ubiquitin-like modifier activating enzyme 5
Gene symbol	Uba5
Other names/aliases	Ube1dc1
Species	Rattus norvegicus
Database cross references - Uba5
ExoCarta	ExoCarta_300968
Entrez Gene	300968
UniProt	Q5M7A4
Uba5 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Uba5

Molecular Function
	ATP binding	GO:0005524	IEA
	UFM1 activating enzyme activity	GO:0071566	ISS
	metal ion binding	GO:0046872	IEA
Biological Process
	protein K69-linked ufmylation	GO:1990592	ISS
	response to endoplasmic reticulum stress	GO:0034976	ISO
	regulation of intracellular estrogen receptor signaling pathway	GO:0033146	ISS
	protein ufmylation	GO:0071569	ISS
Subcellular Localization
	cytoplasm	GO:0005737	ISO
	cytosol	GO:0005829	IBA
	intracellular membrane-bounded organelle	GO:0043231	ISO
	nucleus	GO:0005634	IEA

Experiment description of studies that identified Uba5 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting