Gene description for Uba5
Gene name ubiquitin-like modifier activating enzyme 5
Gene symbol Uba5
Other names/aliases Ube1dc1
Species Rattus norvegicus
 Database cross references - Uba5
ExoCarta ExoCarta_300968
Vesiclepedia VP_300968
Entrez Gene 300968
UniProt Q5M7A4  
 Uba5 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Uba5
Molecular Function
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    ubiquitin-like modifier activating enzyme activity GO:0008641 IEA
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    UFM1 activating enzyme activity GO:0071566 IBA
    UFM1 activating enzyme activity GO:0071566 ISO
    UFM1 activating enzyme activity GO:0071566 ISS
Biological Process
    erythrocyte differentiation GO:0030218 ISO
    erythrocyte differentiation GO:0030218 ISS
    megakaryocyte differentiation GO:0030219 ISO
    megakaryocyte differentiation GO:0030219 ISS
    regulation of type II interferon production GO:0032649 IEA
    regulation of type II interferon production GO:0032649 ISO
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 ISO
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 ISS
    response to endoplasmic reticulum stress GO:0034976 ISO
    response to endoplasmic reticulum stress GO:0034976 ISO
    response to endoplasmic reticulum stress GO:0034976 ISS
    neuromuscular process GO:0050905 ISO
    reticulophagy GO:0061709 ISO
    reticulophagy GO:0061709 ISS
    protein ufmylation GO:0071569 IBA
    protein ufmylation GO:0071569 ISO
    protein ufmylation GO:0071569 ISO
    protein ufmylation GO:0071569 ISS
    protein K69-linked ufmylation GO:1990592 ISO
    protein K69-linked ufmylation GO:1990592 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Uba5 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Uba5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here