Experiment description of studies that identified Shisa5 in exosomes

1 Experiment ID 92 ISEV standards ✔ EM EV Biophysical techniques ✔ HSP70|GAPDH EV Cytosolic markers ✔ CD9|CD151 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M. Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting RatRef-12 Microarray FACS Mass spectrometry [MALDI TOF]

2 Experiment ID 93 ISEV standards ✔ EM EV Biophysical techniques ✔ HSP70|GAPDH EV Cytosolic markers ✔ CD9|CD151 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M. Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS-Tspan8 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting

Protein-protein interactions for Shisa5

Protein Interactor ExoCarta ID Identification method PubMed Species No interactions are found.

Pathways in which Shisa5 is involved

No pathways found