ExoCarta: Gene summary

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Gene description for Thg1l
Gene name	tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
Gene symbol	Thg1l
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Thg1l
ExoCarta	ExoCarta_303067
Vesiclepedia	VP_303067
Entrez Gene	303067
UniProt	Q5M965
Thg1l identified in sEVs derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Thg1l

Molecular Function
	magnesium ion binding	GO:0000287	IEA
	magnesium ion binding	GO:0000287	ISO
	guanyl-nucleotide exchange factor activity	GO:0005085	IEA
	guanyl-nucleotide exchange factor activity	GO:0005085	ISO
	ATP binding	GO:0005524	IEA
	ATP binding	GO:0005524	ISO
	GTP binding	GO:0005525	IEA
	GTP binding	GO:0005525	ISO
	tRNA guanylyltransferase activity	GO:0008193	IBA
	tRNA guanylyltransferase activity	GO:0008193	IEA
	tRNA guanylyltransferase activity	GO:0008193	ISO
	tRNA guanylyltransferase activity	GO:0008193	ISS
	nucleotidyltransferase activity	GO:0016779	ISO
	identical protein binding	GO:0042802	IEA
	identical protein binding	GO:0042802	ISO
Biological Process
	tRNA modification	GO:0006400	IEA
	tRNA modification	GO:0006400	ISS
	response to oxidative stress	GO:0006979	IEA
	response to oxidative stress	GO:0006979	ISO
	response to oxidative stress	GO:0006979	ISS
	tRNA processing	GO:0008033	IBA
	tRNA processing	GO:0008033	ISO
	tRNA processing	GO:0008033	ISS
	mitochondrial fusion	GO:0008053	ISO
	mitochondrial fusion	GO:0008053	ISS
	protein homotetramerization	GO:0051289	IEA
	protein homotetramerization	GO:0051289	ISO
	tRNA 5'-end processing	GO:0099116	IEA
	stress-induced mitochondrial fusion	GO:1990046	IEA
	stress-induced mitochondrial fusion	GO:1990046	ISO
	stress-induced mitochondrial fusion	GO:1990046	ISS
Subcellular Localization
	mitochondrion	GO:0005739	IEA
	mitochondrion	GO:0005739	ISO
	transferase complex	GO:1990234	IEA
	transferase complex	GO:1990234	ISO

Experiment description of studies that identified Thg1l in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP90\|HSC70\|MHCI	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting