Gene description for Irgm
Gene name immunity-related GTPase family, M
Gene symbol Irgm
Other names/aliases Ifggd3
Ifi1
Species Rattus norvegicus
 Database cross references - Irgm
ExoCarta ExoCarta_303090
Vesiclepedia VP_303090
Entrez Gene 303090
UniProt Q6AYC2  
 Irgm identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Irgm
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    G protein activity GO:0003925 ISO
    GTP binding GO:0005525 IEA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
    hydrolase activity GO:0016787 IEA
    protein kinase binding GO:0019901 ISO
    protein-macromolecule adaptor activity GO:0030674 ISO
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 ISO
    protein serine/threonine kinase activator activity GO:0043539 ISO
    CARD domain binding GO:0050700 ISO
    BH3 domain binding GO:0051434 ISO
    cardiolipin binding GO:1901612 ISO
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 ISO
    positive regulation of protein phosphorylation GO:0001934 ISO
    defense response GO:0006952 ISO
    response to bacterium GO:0009617 ISO
    positive regulation of autophagy GO:0010508 ISO
    positive regulation of autophagy GO:0010508 ISS
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 ISO
    protein destabilization GO:0031648 ISO
    negative regulation of type I interferon production GO:0032480 ISO
    negative regulation of type I interferon production GO:0032480 ISS
    negative regulation of type II interferon production GO:0032689 ISO
    negative regulation of type II interferon production GO:0032689 ISS
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    cellular response to interferon-beta GO:0035458 IBA
    defense response to bacterium GO:0042742 ISO
    defense response to bacterium GO:0042742 ISS
    positive regulation of macrophage activation GO:0043032 ISO
    positive regulation of macrophage activation GO:0043032 ISS
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 ISO
    regulation of protein-containing complex assembly GO:0043254 ISO
    innate immune response GO:0045087 IBA
    innate immune response GO:0045087 ISO
    innate immune response GO:0045087 ISS
    negative regulation of defense response to virus GO:0050687 ISO
    negative regulation of defense response to virus GO:0050687 ISS
    negative regulation of inflammatory response GO:0050728 ISO
    negative regulation of inflammatory response GO:0050728 ISS
    protein stabilization GO:0050821 ISO
    defense response to Gram-negative bacterium GO:0050829 ISO
    positive regulation of type II interferon-mediated signaling pathway GO:0060335 ISO
    regulation of protein complex stability GO:0061635 ISO
    protein lipidation involved in autophagosome assembly GO:0061739 ISO
    CAMKK-AMPK signaling cascade GO:0061762 ISO
    protein-containing complex assembly GO:0065003 ISO
    nucleotide-binding oligomerization domain containing 2 signaling pathway GO:0070431 ISO
    protein targeting to vacuole involved in autophagy GO:0071211 ISO
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to type II interferon GO:0071346 ISO
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISO
    regulation of mitochondrial fission GO:0090140 ISO
    regulation of mitochondrial fission GO:0090140 ISS
    positive regulation of mitochondrial fission GO:0090141 ISO
    autophagosome maturation GO:0097352 ISO
    autophagosome maturation GO:0097352 ISS
    negative regulation of cGAS/STING signaling pathway GO:0160049 ISO
    negative regulation of NLRP3 inflammasome complex assembly GO:1900226 ISO
    negative regulation of NLRP3 inflammasome complex assembly GO:1900226 ISS
    positive regulation of autophagosome maturation GO:1901098 ISO
    positive regulation of mitophagy GO:1901526 ISO
    positive regulation of mitophagy GO:1901526 ISS
    negative regulation of hematopoietic stem cell proliferation GO:1902034 ISO
    negative regulation of hematopoietic stem cell proliferation GO:1902034 ISS
    positive regulation of xenophagy GO:1904417 ISO
    positive regulation of lysosome organization GO:1905673 ISO
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 ISO
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 ISS
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    autophagosome membrane GO:0000421 ISO
    phagocytic cup GO:0001891 IEA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISO
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    late endosome GO:0005770 ISO
    autophagosome GO:0005776 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 ISO
    membrane GO:0016020 IEA
    phagocytic vesicle membrane GO:0030670 IEA
    late endosome membrane GO:0031902 IEA
    mitochondrial membrane GO:0031966 ISO
    mitochondrial membrane GO:0031966 ISS
    cell projection GO:0042995 IEA
    autolysosome GO:0044754 ISO
    phagocytic vesicle GO:0045335 ISO
 Experiment description of studies that identified Irgm in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Irgm
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Irgm is involved
No pathways found





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