Gene description for Map2k3
Gene name mitogen activated protein kinase kinase 3
Gene symbol Map2k3
Other names/aliases Mek3
Mkk3
Species Rattus norvegicus
 Database cross references - Map2k3
ExoCarta ExoCarta_303200
Vesiclepedia VP_303200
Entrez Gene 303200
 Map2k3 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Map2k3
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IEA
    MAP kinase kinase activity GO:0004708 IBA
    MAP kinase kinase activity GO:0004708 ISO
    protein tyrosine kinase activity GO:0004713 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 ISO
Biological Process
    MAPK cascade GO:0000165 IBA
    MAPK cascade GO:0000165 ISO
    regulation of cytokine production GO:0001817 ISO
    positive regulation of protein phosphorylation GO:0001934 IDA
    response to ischemia GO:0002931 IEP
    inflammatory response GO:0006954 ISO
    heart development GO:0007507 IEP
    stress-activated protein kinase signaling cascade GO:0031098 ISO
    negative regulation of hippo signaling GO:0035331 ISO
    cellular response to vascular endothelial growth factor stimulus GO:0035924 ISO
    p38MAPK cascade GO:0038066 ISO
    positive regulation of MAPK cascade GO:0043410 ISO
    positive regulation of blood vessel endothelial cell migration GO:0043536 ISO
    positive regulation of protein kinase activity GO:0045860 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISO
    cardiac muscle contraction GO:0060048 ISO
    cellular response to lipopolysaccharide GO:0071222 IDA
    cellular response to sorbitol GO:0072709 IEP
 Experiment description of studies that identified Map2k3 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Map2k3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mapk14  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Map2k3 is involved
No pathways found





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