Gene description for Wdr81
Gene name WD repeat domain 81
Gene symbol Wdr81
Other names/aliases RGD1311334
Species Rattus norvegicus
 Database cross references - Wdr81
ExoCarta ExoCarta_303312
Vesiclepedia VP_303312
Entrez Gene 303312
UniProt D4A929  
 Wdr81 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Wdr81
Molecular Function
    phosphatidylinositol 3-kinase regulator activity GO:0035014 IBA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 IEA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 ISO
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 ISS
    phosphatidylinositol 3-kinase inhibitor activity GO:0141039 IEA
    phosphatidylinositol 3-kinase inhibitor activity GO:0141039 ISO
    phosphatidylinositol 3-kinase inhibitor activity GO:0141039 ISS
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    mitochondrion organization GO:0007005 IEA
    mitochondrion organization GO:0007005 ISO
    mitochondrion organization GO:0007005 ISS
    aggrephagy GO:0035973 IBA
    aggrephagy GO:0035973 IEA
    aggrephagy GO:0035973 ISO
    aggrephagy GO:0035973 ISS
    early endosome to late endosome transport GO:0045022 IEA
    early endosome to late endosome transport GO:0045022 ISO
    early endosome to late endosome transport GO:0045022 ISS
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 IEA
    autophagosome membrane GO:0000421 ISO
    autophagosome membrane GO:0000421 ISS
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    endoplasmic reticulum membrane GO:0005789 ISO
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    endosome membrane GO:0010008 ISO
    endosome membrane GO:0010008 ISS
    early endosome membrane GO:0031901 IEA
    early endosome membrane GO:0031901 ISO
    early endosome membrane GO:0031901 ISS
    late endosome membrane GO:0031902 IEA
    late endosome membrane GO:0031902 ISO
    late endosome membrane GO:0031902 ISS
 Experiment description of studies that identified Wdr81 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Wdr81
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Wdr81 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here