Gene description for Nle1
Gene name notchless homolog 1 (Drosophila)
Gene symbol Nle1
Other names/aliases RGD1308301
Species Rattus norvegicus
 Database cross references - Nle1
ExoCarta ExoCarta_303372
Entrez Gene 303372
 Nle1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Nle1
Biological Process
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:2001268 ISO
    skeletal system morphogenesis GO:0048705 ISO
    ribosomal large subunit biogenesis GO:0042273 ISO
    negative regulation of mitotic cell cycle GO:0045930 ISO
    kidney development GO:0001822 ISO
    inner cell mass cell differentiation GO:0001826 ISO
    hematopoietic stem cell homeostasis GO:0061484 ISO
    somitogenesis GO:0001756 ISO
    Notch signaling pathway GO:0007219 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
Subcellular Localization
    nucleolus GO:0005730 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Nle1 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nle1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nle1 is involved
No pathways found





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