Gene description for Lrrc8d
Gene name leucine rich repeat containing 8 family, member D
Gene symbol Lrrc8d
Other names/aliases Lrrc5
Species Rattus norvegicus
 Database cross references - Lrrc8d
ExoCarta ExoCarta_305131
Vesiclepedia VP_305131
Entrez Gene 305131
UniProt Q5U308  
 Lrrc8d identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Lrrc8d
Molecular Function
    volume-sensitive anion channel activity GO:0005225 IEA
    volume-sensitive anion channel activity GO:0005225 IMP
    volume-sensitive anion channel activity GO:0005225 ISO
    volume-sensitive anion channel activity GO:0005225 ISO
    volume-sensitive anion channel activity GO:0005225 ISS
Biological Process
    intracellular glucose homeostasis GO:0001678 IEA
    intracellular glucose homeostasis GO:0001678 ISO
    intracellular glucose homeostasis GO:0001678 ISS
    taurine transmembrane transport GO:0015734 IEA
    taurine transmembrane transport GO:0015734 IMP
    taurine transmembrane transport GO:0015734 ISO
    taurine transmembrane transport GO:0015734 ISS
    aspartate transmembrane transport GO:0015810 IBA
    aspartate transmembrane transport GO:0015810 IEA
    aspartate transmembrane transport GO:0015810 IMP
    aspartate transmembrane transport GO:0015810 ISO
    aspartate transmembrane transport GO:0015810 ISS
    protein hexamerization GO:0034214 IEA
    protein hexamerization GO:0034214 ISO
    protein hexamerization GO:0034214 ISS
    intracellular signal transduction GO:0035556 IBA
    cellular response to osmotic stress GO:0071470 IEA
    cellular response to osmotic stress GO:0071470 IMP
    cellular response to osmotic stress GO:0071470 ISO
    cellular response to osmotic stress GO:0071470 ISS
    monoatomic anion transmembrane transport GO:0098656 IBA
    monoatomic anion transmembrane transport GO:0098656 IEA
    monoatomic anion transmembrane transport GO:0098656 ISO
    monoatomic anion transmembrane transport GO:0098656 ISS
    cyclic-GMP-AMP transmembrane import across plasma membrane GO:0140361 ISO
    cyclic-GMP-AMP transmembrane import across plasma membrane GO:0140361 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum membrane GO:0005789 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    monoatomic ion channel complex GO:0034702 IBA
    monoatomic ion channel complex GO:0034702 IEA
    monoatomic ion channel complex GO:0034702 IMP
    monoatomic ion channel complex GO:0034702 ISO
    monoatomic ion channel complex GO:0034702 ISS
 Experiment description of studies that identified Lrrc8d in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Lrrc8d
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Lrrc8d is involved
PathwayEvidenceSource
Miscellaneous transport and binding events IEA Reactome
Transport of small molecules IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here