Gene description for Eif6
Gene name eukaryotic translation initiation factor 6
Gene symbol Eif6
Other names/aliases Itgb4bp
eIF-6
Species Rattus norvegicus
 Database cross references - Eif6
ExoCarta ExoCarta_305506
Vesiclepedia VP_305506
Entrez Gene 305506
UniProt Q3KRD8  
 Eif6 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Eif6
Molecular Function
    translation initiation factor activity GO:0003743 IEA
    ribosome binding GO:0043022 ISO
    ribosomal large subunit binding GO:0043023 IBA
    ribosomal large subunit binding GO:0043023 IEA
Biological Process
    ribosomal subunit export from nucleus GO:0000054 IBA
    ribosomal subunit export from nucleus GO:0000054 IEA
    maturation of 5.8S rRNA GO:0000460 IBA
    maturation of LSU-rRNA GO:0000470 IBA
    regulation of glycolytic process GO:0006110 ISO
    regulation of glycolytic process GO:0006110 ISS
    translational initiation GO:0006413 IEA
    biological_process GO:0008150 ND
    response to insulin GO:0032868 ISO
    response to insulin GO:0032868 ISS
    miRNA-mediated post-transcriptional gene silencing GO:0035195 ISO
    miRNA-mediated post-transcriptional gene silencing GO:0035195 ISS
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 ISO
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 ISS
    cytosolic ribosome assembly GO:0042256 IEA
    cytosolic ribosome assembly GO:0042256 ISO
    ribosomal large subunit biogenesis GO:0042273 IEA
    regulation of fatty acid biosynthetic process GO:0042304 ISO
    regulation of fatty acid biosynthetic process GO:0042304 ISS
    regulation of megakaryocyte differentiation GO:0045652 ISO
    regulation of megakaryocyte differentiation GO:0045652 ISS
    positive regulation of translation GO:0045727 ISO
    positive regulation of translation GO:0045727 ISS
    assembly of large subunit precursor of preribosome GO:1902626 IBA
    regulation of reactive oxygen species metabolic process GO:2000377 ISO
    regulation of reactive oxygen species metabolic process GO:2000377 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    lamin filament GO:0005638 ISO
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    intermediate filament GO:0005882 ISO
    synapse GO:0045202 ISO
 Experiment description of studies that identified Eif6 in sEVs
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Eif6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sumo3  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Eif6 is involved
No pathways found





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