Gene description for Pde12
Gene name phosphodiesterase 12
Gene symbol Pde12
Other names/aliases LRRGT00074
RGD1310975
Species Rattus norvegicus
 Database cross references - Pde12
ExoCarta ExoCarta_306231
Vesiclepedia VP_306231
Entrez Gene 306231
 Pde12 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Pde12
Molecular Function
    3'-5'-RNA exonuclease activity GO:0000175 IBA
    3'-5'-RNA exonuclease activity GO:0000175 ISO
    catalytic activity GO:0003824 IEA
    exonuclease activity GO:0004527 ISO
    poly(A)-specific ribonuclease activity GO:0004535 IEA
    poly(A)-specific ribonuclease activity GO:0004535 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 IBA
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 IEA
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 ISO
    mitochondrial mRNA catabolic process GO:0000958 IEA
    mitochondrial mRNA catabolic process GO:0000958 ISO
    mRNA processing GO:0006397 IEA
    cellular response to interferon-alpha GO:0035457 IEA
    cellular response to interferon-alpha GO:0035457 ISO
    regulation of mitochondrial mRNA stability GO:0044528 IEA
    regulation of mitochondrial mRNA stability GO:0044528 ISO
    positive regulation of viral genome replication GO:0045070 IEA
    positive regulation of viral genome replication GO:0045070 ISO
    cellular response to type II interferon GO:0071346 IEA
    cellular response to type II interferon GO:0071346 ISO
    cellular response to dsRNA GO:0071359 IEA
    cellular response to dsRNA GO:0071359 ISO
    antiviral innate immune response GO:0140374 IEA
    antiviral innate immune response GO:0140374 ISO
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IEA
    mitochondrial matrix GO:0005759 ISO
 Experiment description of studies that identified Pde12 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pde12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pde12 is involved
No pathways found





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