Gene description for Pde12
Gene name phosphodiesterase 12
Gene symbol Pde12
Other names/aliases LRRGT00074
RGD1310975
Species Rattus norvegicus
 Database cross references - Pde12
ExoCarta ExoCarta_306231
Entrez Gene 306231
 Pde12 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Pde12
Molecular Function
    poly(A)-specific ribonuclease activity GO:0004535 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    mRNA processing GO:0006397 IEA
    RNA phosphodiester bond hydrolysis, exonucleolytic GO:0090503 IEA
Subcellular Localization
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IEA
 Experiment description of studies that identified Pde12 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pde12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pde12 is involved
No pathways found





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