ExoCarta: Gene summary

Credits | Contact

Gene description for Me2
Gene name	malic enzyme 2, NAD(+)-dependent, mitochondrial
Gene symbol	Me2
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Me2
ExoCarta	ExoCarta_307270
Entrez Gene	307270
Me2 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Me2

Molecular Function
	NAD binding	GO:0051287	IEA
	malic enzyme activity	GO:0004470	ISO
	metal ion binding	GO:0046872	IEA
	malate dehydrogenase (decarboxylating) (NAD+) activity	GO:0004471	IEA
Biological Process
	regulation of NADP metabolic process	GO:1902031	ISO
	oxidation-reduction process	GO:0055114	IEA
	malate metabolic process	GO:0006108	IEA
Subcellular Localization
	intracellular membrane-bounded organelle	GO:0043231	ISO
	mitochondrion	GO:0005739	ISO

Experiment description of studies that identified Me2 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting