Gene description for Hars
Gene name histidyl-tRNA synthetase
Gene symbol Hars
Other names/aliases Dnd1
Species Rattus norvegicus
 Database cross references - Hars
ExoCarta ExoCarta_307492
Vesiclepedia VP_307492
Entrez Gene 307492
 Hars identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hars
Molecular Function
    RNA binding GO:0003723 IBA
    histidine-tRNA ligase activity GO:0004821 IBA
    histidine-tRNA ligase activity GO:0004821 ISO
    ATP binding GO:0005524 ISO
    identical protein binding GO:0042802 IBA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
Biological Process
    histidyl-tRNA aminoacylation GO:0006427 IBA
    histidyl-tRNA aminoacylation GO:0006427 ISO
    mitochondrial translation GO:0032543 IBA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Hars in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hars
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hars is involved
No pathways found





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