|
 Gene description for Eif5b |
| Gene name |
eukaryotic translation initiation factor 5B |
| Gene symbol |
Eif5b |
| Other names/aliases |
- |
| Species |
Rattus norvegicus |
| Database cross references - Eif5b |
| ExoCarta |
ExoCarta_308306 |
| Entrez Gene |
308306 |
| UniProt |
B2GUV7
|
| Eif5b identified in exosomes derived from the following tissue/cell type |
|
Reticulocytes
|
21828046
|
|
Reticulocytes
|
21828046
|
| Gene ontology annotations for Eif5b |
|
|
| Experiment description of studies that identified Eif5b in exosomes |
| 1 |
| Experiment ID |
97 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
|
✔
MHCI
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [Orbitrap]
|
| PubMed ID |
21828046
|
| Organism |
Rattus norvegicus |
| Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
| Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
| Journal name |
JBC
|
| Publication year |
2011 |
| Sample |
Reticulocytes |
| Sample name |
Reticulocytes - D4 Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.16-1.21 g/mL
|
| Molecules identified in the study |
Protein
Lipids |
| Methods used in the study |
Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting |
|
|
| 2 |
| Experiment ID |
98 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|HSP90|HSC70
|
EV Cytosolic markers |
|
✔
MHCI
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [Orbitrap]
|
| PubMed ID |
21828046
|
| Organism |
Rattus norvegicus |
| Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
| Authors |
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. |
| Journal name |
JBC
|
| Publication year |
2011 |
| Sample |
Reticulocytes |
| Sample name |
Reticulocytes - D7 Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.25 g/mL
|
| Molecules identified in the study |
Protein
Lipids |
| Methods used in the study |
Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting |
|
|
| Protein-protein interactions for Eif5b |
| |
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
|
No interactions are found.
|
|
| Pathways in which Eif5b is involved |
|
|
|
