Gene description for Pnkp
Gene name polynucleotide kinase 3'-phosphatase
Gene symbol Pnkp
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Pnkp
ExoCarta ExoCarta_308576
Vesiclepedia VP_308576
Entrez Gene 308576
 Pnkp identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Pnkp
Molecular Function
    polynucleotide 3'-phosphatase activity GO:0046403 IBA
    polynucleotide 3'-phosphatase activity GO:0046403 ISO
    ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity GO:0046404 IBA
    ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity GO:0046404 ISO
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 ISO
Biological Process
    DNA repair GO:0006281 IBA
    DNA repair GO:0006281 ISO
    double-strand break repair via nonhomologous end joining GO:0006303 ISO
    DNA damage response GO:0006974 ISO
    response to oxidative stress GO:0006979 ISO
    biological_process GO:0008150 ND
    positive regulation of telomere maintenance GO:0032206 ISO
    DNA ligation involved in DNA repair GO:0051103 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    positive regulation of double-strand break repair via nonhomologous end joining GO:2001034 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    SCF ubiquitin ligase complex GO:0019005 ISO
    site of double-strand break GO:0035861 ISO
 Experiment description of studies that identified Pnkp in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pnkp
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pnkp is involved
No pathways found





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