Gene description for Pnkp
Gene name polynucleotide kinase 3'-phosphatase
Gene symbol Pnkp
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Pnkp
ExoCarta ExoCarta_308576
Entrez Gene 308576
 Pnkp identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Pnkp
Molecular Function
    molecular_function GO:0003674 ND
    polynucleotide 3'-phosphatase activity GO:0046403 ISO
    ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity GO:0046404 ISO
    double-stranded DNA binding GO:0003690 IBA
    nucleotide kinase activity GO:0019201 ISO
Biological Process
    dephosphorylation GO:0016311 ISO
    response to oxidative stress GO:0006979 ISO
    DNA repair GO:0006281 ISO
    polynucleotide 3' dephosphorylation GO:0098506 ISO
    nucleotide phosphorylation GO:0046939 ISO
    biological_process GO:0008150 ND
    DNA damage response, detection of DNA damage GO:0042769 ISO
    DNA 3' dephosphorylation involved in DNA repair GO:0098504 IBA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    nucleolus GO:0005730 ISO
    nucleus GO:0005634 ISO
    membrane GO:0016020 ISO
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Pnkp in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pnkp
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pnkp is involved
No pathways found





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