Gene description for Rhog
Gene name ras homolog family member G
Gene symbol Rhog
Other names/aliases Arhg
Species Rattus norvegicus
 Database cross references - Rhog
ExoCarta ExoCarta_308875
Vesiclepedia VP_308875
Entrez Gene 308875
 Rhog identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Rhog
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTP binding GO:0005525 IBA
    protein kinase binding GO:0019901 IBA
Biological Process
    actin filament organization GO:0007015 IBA
    establishment or maintenance of cell polarity GO:0007163 IBA
    Rho protein signal transduction GO:0007266 ISO
    regulation of cell shape GO:0008360 IBA
    Rac protein signal transduction GO:0016601 IBA
    Rac protein signal transduction GO:0016601 ISO
    actin cytoskeleton organization GO:0030036 ISO
    cortical cytoskeleton organization GO:0030865 IBA
    regulation of actin cytoskeleton organization GO:0032956 IBA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    cell chemotaxis GO:0060326 IBA
    cell chemotaxis GO:0060326 ISO
    activation of GTPase activity GO:0090630 ISO
    regulation of postsynapse assembly GO:0150052 ISO
    regulation of ruffle assembly GO:1900027 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
Subcellular Localization
    cytoskeleton GO:0005856 IBA
    plasma membrane GO:0005886 IBA
    cytoplasmic vesicle GO:0031410 IBA
    cell projection GO:0042995 IBA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Rhog in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rhog
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rhog is involved
No pathways found





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