Gene description for Itgal
Gene name integrin, alpha L
Gene symbol Itgal
Other names/aliases LFA-1
Species Rattus norvegicus
 Database cross references - Itgal
ExoCarta ExoCarta_308995
Vesiclepedia VP_308995
Entrez Gene 308995
 Itgal identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Itgal
Molecular Function
    integrin binding GO:0005178 IBA
    ICAM-3 receptor activity GO:0030369 ISO
    protein-containing complex binding GO:0044877 IPI
    metal ion binding GO:0046872 IEA
    cell adhesion molecule binding GO:0050839 ISO
Biological Process
    T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:0002291 ISO
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
    cell-matrix adhesion GO:0007160 IBA
    cell-matrix adhesion GO:0007160 ISO
    cell surface receptor signaling pathway GO:0007166 ISO
    integrin-mediated signaling pathway GO:0007229 IBA
    calcium-mediated signaling GO:0019722 ISO
    regulation of cell-cell adhesion GO:0022407 ISO
    positive regulation of cell-cell adhesion GO:0022409 ISO
    cell adhesion mediated by integrin GO:0033627 IBA
    memory T cell extravasation GO:0035683 ISO
    positive regulation of T cell proliferation GO:0042102 ISO
    receptor clustering GO:0043113 ISO
    activated T cell proliferation GO:0050798 ISO
    positive regulation of calcium-mediated signaling GO:0050850 ISO
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion GO:0098609 IMP
    cell-cell adhesion GO:0098609 ISO
Subcellular Localization
    immunological synapse GO:0001772 ISO
    cell-cell junction GO:0005911 ISO
    integrin complex GO:0008305 IBA
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 ISO
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    integrin alphaL-beta2 complex GO:0034687 ISO
 Experiment description of studies that identified Itgal in sEVs
1
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Itgal
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Itgal is involved
No pathways found





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