Gene description for Itgb2
Gene name integrin, beta 2
Gene symbol Itgb2
Other names/aliases Cd18
Species Rattus norvegicus
 Database cross references - Itgb2
ExoCarta ExoCarta_309684
Vesiclepedia VP_309684
Entrez Gene 309684
 Itgb2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Itgb2
Molecular Function
    protein kinase binding GO:0019901 ISO
    glycoprotein binding GO:0001948 ISO
    ICAM-3 receptor activity GO:0030369 ISO
    protein complex binding GO:0032403 IPI
    protein heterodimerization activity GO:0046982 IPI
    cell adhesion molecule binding GO:0050839 ISO
Biological Process
    heterotypic cell-cell adhesion GO:0034113 ISO
    cell-matrix adhesion GO:0007160 ISO
    activated T cell proliferation GO:0050798 ISO
    positive regulation of angiogenesis GO:0045766 IMP
    natural killer cell activation GO:0030101 IMP
    endothelial cell migration GO:0043542 IMP
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 ISO
    leukocyte migration involved in inflammatory response GO:0002523 IMP
    positive regulation of nitric oxide biosynthetic process GO:0045429 IMP
    neutrophil chemotaxis GO:0030593 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    single organismal cell-cell adhesion GO:0016337 ISO
    cell adhesion GO:0007155 ISO
    aging GO:0007568 IEP
    cellular extravasation GO:0045123 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
    endodermal cell differentiation GO:0035987 ISO
    receptor clustering GO:0043113 ISO
    integrin-mediated signaling pathway GO:0007229 ISO
Subcellular Localization
    receptor complex GO:0043235 ISO
    plasma membrane GO:0005886 IDA
    extracellular exosome GO:0070062 ISO
    extracellular vesicle GO:1903561 ISO
    integrin alphaL-beta2 complex GO:0034687 ISO
    membrane GO:0016020 ISO
    membrane raft GO:0045121 ISO
    cell surface GO:0009986 ISO
 Experiment description of studies that identified Itgb2 in exosomes
1
Experiment ID 94
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 99
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 100
MISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Itgb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Itgb2 is involved
No pathways found





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