Gene description for Ap3b1
Gene name adaptor-related protein complex 3, beta 1 subunit
Gene symbol Ap3b1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ap3b1
ExoCarta ExoCarta_309969
Vesiclepedia VP_309969
Entrez Gene 309969
 Ap3b1 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Ap3b1
Molecular Function
    protein phosphatase binding GO:0019903 ISO
    GTP-dependent protein binding GO:0030742 ISO
Biological Process
    cell morphogenesis GO:0000902 ISO
    toll-like receptor signaling pathway GO:0002224 ISO
    hematopoietic progenitor cell differentiation GO:0002244 ISO
    respiratory system process GO:0003016 ISO
    protein targeting GO:0006605 ISO
    protein targeting to lysosome GO:0006622 ISO
    intracellular zinc ion homeostasis GO:0006882 ISO
    intracellular protein transport GO:0006886 ISO
    inflammatory response GO:0006954 ISO
    lysosome organization GO:0007040 ISO
    spermatogenesis GO:0007283 ISO
    single fertilization GO:0007338 ISO
    blood coagulation GO:0007596 ISO
    anterograde axonal transport GO:0008089 ISO
    biological_process GO:0008150 ND
    vesicle-mediated transport GO:0016192 IBA
    antigen processing and presentation GO:0019882 ISO
    lung development GO:0030324 ISO
    granulocyte differentiation GO:0030851 ISO
    melanosome organization GO:0032438 ISO
    melanosome organization GO:0032438 ISO
    protein localization to cell surface GO:0034394 ISO
    protein modification process GO:0036211 ISO
    mRNA transcription by RNA polymerase II GO:0042789 ISO
    pigmentation GO:0043473 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    antigen processing and presentation, exogenous lipid antigen via MHC class Ib GO:0048007 ISO
    anterograde synaptic vesicle transport GO:0048490 IBA
    anterograde synaptic vesicle transport GO:0048490 ISO
    homeostasis of number of cells GO:0048872 ISO
    regulation of catalytic activity GO:0050790 ISO
    positive regulation of NK T cell differentiation GO:0051138 ISO
    platelet dense granule organization GO:0060155 ISO
    lung morphogenesis GO:0060425 ISO
    membrane organization GO:0061024 ISO
    establishment of protein localization to mitochondrial membrane involved in mitochondrial fission GO:0090152 ISO
    skin epidermis development GO:0098773 ISO
Subcellular Localization
    mitochondrion GO:0005739 IEA
    AP-3 adaptor complex GO:0030123 IEA
    clathrin adaptor complex GO:0030131 IEA
    clathrin-coated vesicle membrane GO:0030665 IEA
    axon cytoplasm GO:1904115 IEA
    microvesicle GO:1990742 ISO
 Experiment description of studies that identified Ap3b1 in sEVs
1
Experiment ID 92
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Ap3b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pi4k2a  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ap3b1 is involved
No pathways found





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