Gene description for Psmd14
Gene name proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
Gene symbol Psmd14
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Psmd14
ExoCarta ExoCarta_311078
Vesiclepedia VP_311078
Entrez Gene 311078
 Psmd14 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Psmd14
Molecular Function
    endopeptidase activator activity GO:0061133 ISO
    proteasome binding GO:0070628 IBA
    proteasome binding GO:0070628 ISO
    metal-dependent deubiquitinase activity GO:0140492 IBA
    metal-dependent deubiquitinase activity GO:0140492 ISO
Biological Process
    double-strand break repair via homologous recombination GO:0000724 ISO
    double-strand break repair via nonhomologous end joining GO:0006303 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    response to ethanol GO:0045471 IEP
    regulation of proteasomal protein catabolic process GO:0061136 ISO
    protein K63-linked deubiquitination GO:0070536 ISO
Subcellular Localization
    proteasome complex GO:0000502 ISO
    nucleus GO:0005634 IBA
    proteasome regulatory particle, lid subcomplex GO:0008541 IBA
    proteasome regulatory particle, lid subcomplex GO:0008541 ISO
    proteasome accessory complex GO:0022624 ISO
    cytosolic proteasome complex GO:0031597 IDA
 Experiment description of studies that identified Psmd14 in sEVs
1
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Psmd14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C2  
Affinity Capture-MS Rattus norvegicus
2 Mib1  
Affinity Capture-MS Rattus norvegicus
3 Rad23b  
Affinity Capture-MS Rattus norvegicus
4 Ublcp1  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Psmd14 is involved
No pathways found





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