Gene description for Itpa
Gene name inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
Gene symbol Itpa
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Itpa
ExoCarta ExoCarta_311422
Vesiclepedia VP_311422
Entrez Gene 311422
UniProt D3ZW55  
 Itpa identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Itpa
Molecular Function
    nucleotide binding GO:0000166 IEA
    dITP diphosphatase activity GO:0035870 ISO
    ITP diphosphatase activity GO:0036220 IEA
    XTP diphosphatase activity GO:0036222 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    nucleoside triphosphate diphosphatase activity GO:0047429 IBA
    nucleoside triphosphate diphosphatase activity GO:0047429 IEA
    nucleoside triphosphate diphosphatase activity GO:0047429 ISO
    8-oxo-(d)RTP hydrolase activity GO:0106379 IEA
Biological Process
    ITP catabolic process GO:0006193 ISO
    biological_process GO:0008150 ND
    nucleotide metabolic process GO:0009117 IEA
    nucleoside triphosphate catabolic process GO:0009143 IBA
    nucleoside triphosphate catabolic process GO:0009143 IEA
    deoxyribonucleoside triphosphate catabolic process GO:0009204 IEA
    chromosome organization GO:0051276 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Itpa in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Itpa
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Itpa is involved
PathwayEvidenceSource
Drug ADME IEA Reactome
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome
Nucleotide catabolism IEA Reactome
Purine catabolism IEA Reactome
Ribavirin ADME IEA Reactome





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