ExoCarta: Gene summary

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Gene description for Mrrf
Gene name	mitochondrial ribosome recycling factor
Gene symbol	Mrrf
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Mrrf
ExoCarta	ExoCarta_311903
Vesiclepedia	VP_311903
Entrez Gene	311903
UniProt	Q5RKI9
Mrrf identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells	20124479
Pancreatic cancer cells	20124479

Gene ontology annotations for Mrrf

Molecular Function
	ribosomal large subunit binding	GO:0043023	IBA
Biological Process
	translation	GO:0006412	IBA
	translation	GO:0006412	IEA
	ribosome disassembly	GO:0032790	ISO
	ribosome disassembly	GO:0032790	ISS
Subcellular Localization
	mitochondrion	GO:0005739	IBA
	mitochondrion	GO:0005739	ISO
	mitochondrion	GO:0005739	ISO
	mitochondrion	GO:0005739	ISS

Experiment description of studies that identified Mrrf in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ HSP70\|GAPDH\|CD9\|CD151	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

mrna

Identification method

Microarray

PubMed ID

20124479

Organism

Rattus norvegicus

Experiment description

Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.

Authors

"Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."

Journal name

Publication year

2010

Sample

Pancreatic cancer cells

Sample name

Isolation/purification methods

Differential centrifugation
Sucrose density gradient

Flotation density

1.14-1.17 g/mL

Molecules identified in the study

Protein
mRNA

Methods used in the study

RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]