Gene description for Vinc
Gene name Vinculin
Gene symbol Vinc
Other names/aliases CG3299
CT11081
DmelCG3299
Dmvincp
EG:103B4.1
Vin
Vin2EF
vinc
Species Drosophila melanogaster
 Database cross references - Vinc
ExoCarta ExoCarta_31201
Vesiclepedia VP_31201
Entrez Gene 31201
UniProt O46037  
 Vinc identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Vinc
Subcellular Localization
    focal adhesion GO:0005925 ISS
    cytoplasmic side of apical plasma membrane GO:0098592 IDA
 Experiment description of studies that identified Vinc in exosomes
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Vinc
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hip1  
Two-hybrid Drosophila melanogaster
2 Su(H)  
Two-hybrid Drosophila melanogaster
3 CG42748  
Two-hybrid Drosophila melanogaster
4 CAP  
Two-hybrid Drosophila melanogaster
Two-hybrid Drosophila melanogaster
5 CG4095  
Two-hybrid Drosophila melanogaster
6 cry  
Proximity Label-MS Drosophila melanogaster
7 CG8547  
Two-hybrid Drosophila melanogaster
8 Pax  
Two-hybrid Drosophila melanogaster
Two-hybrid Drosophila melanogaster
9 FK506-bp2  
Two-hybrid Drosophila melanogaster
10 ND75  
Two-hybrid Drosophila melanogaster
11 Exo70  
Two-hybrid Drosophila melanogaster
12 Vamp7  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml



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