ExoCarta: Gene summary

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Gene description for Tmem106b
Gene name	transmembrane protein 106B
Gene symbol	Tmem106b
Other names/aliases	LRRGT00101
Species	Rattus norvegicus
Database cross references - Tmem106b
ExoCarta	ExoCarta_312132
Entrez Gene	312132
UniProt	Q6AYA5
Tmem106b identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Tmem106b

Molecular Function
	molecular_function	GO:0003674	ND
	protein binding	GO:0005515	IPI
Biological Process
	dendrite morphogenesis	GO:0048813	ISS
	biological_process	GO:0008150	ND
	transport	GO:0006810	IEA
	lysosome localization	GO:0032418	ISS
Subcellular Localization
	extracellular exosome	GO:0070062	ISO
	cellular_component	GO:0005575	ND
	lysosomal membrane	GO:0005765	ISS
	integral component of membrane	GO:0016021	IEA
	late endosome membrane	GO:0031902	IEA
	intracellular membrane-bounded organelle	GO:0043231	ISO

Experiment description of studies that identified Tmem106b in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ HSC70\|HSP90\|TSG101	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting