Gene description for Tspan9
Gene name tetraspanin 9
Gene symbol Tspan9
Other names/aliases RGD1304740
Species Rattus norvegicus
 Database cross references - Tspan9
ExoCarta ExoCarta_312728
Vesiclepedia VP_312728
Entrez Gene 312728
 Tspan9 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tspan9
Biological Process
    neutrophil homeostasis GO:0001780 ISO
    collagen-activated tyrosine kinase receptor signaling pathway GO:0038063 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    regulation of mitochondrial membrane potential GO:0051881 ISO
    platelet aggregation GO:0070527 ISO
    receptor diffusion trapping GO:0098953 ISO
    mitocytosis GO:0160040 ISO
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 ISO
    tetraspanin-enriched microdomain GO:0097197 ISO
    migrasome GO:0140494 ISO
 Experiment description of studies that identified Tspan9 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tspan9
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tspan9 is involved
No pathways found





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