Gene description for HLF
Gene name hepatic leukemia factor
Gene symbol HLF
Other names/aliases -
Species Homo sapiens
 Database cross references - HLF
ExoCarta ExoCarta_3131
Vesiclepedia VP_3131
Entrez Gene 3131
HGNC 4977
MIM 142385
UniProt Q16534  
 HLF identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for HLF
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IMP
    DNA binding GO:0003677 TAS
    double-stranded DNA binding GO:0003690 TAS
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 IMP
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    regulation of transcription by RNA polymerase II GO:0006357 NAS
    skeletal muscle cell differentiation GO:0035914 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    rhythmic process GO:0048511 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IPI
 Experiment description of studies that identified HLF in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for HLF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNF4G  
Two-hybrid Homo sapiens
2 MYB  
Two-hybrid Homo sapiens
3 DBP  
Two-hybrid Homo sapiens
4 TLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HLF is involved
No pathways found





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