Gene description for Rala
Gene name Ras-like protein A
Gene symbol Rala
Other names/aliases 24639550
CG2849
D-RalA
DRal
DRala
Dm RalA
DmelCG2849
Dral
DralA
Drala
P48555
RALA
Ral
Ral-a
RalA
anon-EST:fe1H4
dRal
l(1)G0174
l(1)G0373
l(1)G0501
ral
ralA
Species Drosophila melanogaster
 Database cross references - Rala
ExoCarta ExoCarta_31332
Vesiclepedia VP_31332
Entrez Gene 31332
UniProt P48555  
 Rala identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Rala
Molecular Function
    GTPase activity GO:0003924 IDA
    protein binding GO:0005515 IPI
    PDZ domain binding GO:0030165 IPI
Biological Process
    Ras protein signal transduction GO:0007265 IGI
    border follicle cell migration GO:0007298 IMP
    R3/R4 cell fate commitment GO:0007464 IMP
    regulation of cell morphogenesis GO:0022604 IMP
    receptor internalization GO:0031623 IMP
    innate immune response GO:0045087 IDA
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IMP
    negative regulation of innate immune response GO:0045824 HMP
    negative regulation of JNK cascade GO:0046329 IGI
    defense response to Gram-negative bacterium GO:0050829 HMP
    positive regulation of stem cell proliferation GO:2000648 IMP
Subcellular Localization
    plasma membrane GO:0005886 HDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified Rala in sEVs
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rala
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 cry  
Proximity Label-MS Drosophila melanogaster
2 Exo84  
Two-hybrid Drosophila melanogaster
3 metl  
Two-hybrid Drosophila melanogaster
4 CG13367  
Two-hybrid Drosophila melanogaster
5 CG11076  
Two-hybrid Drosophila melanogaster
6 Sec5  
Two-hybrid Drosophila melanogaster
7 Rlip  
Two-hybrid Drosophila melanogaster
Two-hybrid Drosophila melanogaster
8 Ank2  
Two-hybrid Drosophila melanogaster
9 vimar  
Two-hybrid Drosophila melanogaster
10 Tom20  
Proximity Label-MS Drosophila melanogaster
11 CHORD  
Two-hybrid Drosophila melanogaster
12 Wnt5  
Two-hybrid Drosophila melanogaster
13 sisA  
Two-hybrid Drosophila melanogaster
14 eIF3-S9  
Two-hybrid Drosophila melanogaster
15 ey  
Two-hybrid Drosophila melanogaster
16 GEFmeso  
Two-hybrid Drosophila melanogaster
17 zuc  
Proximity Label-MS Drosophila melanogaster
18 CG32532  
Two-hybrid Drosophila melanogaster
19 CG12470  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Rala is involved
No pathways found





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