Gene description for Ubr4
Gene name ubiquitin protein ligase E3 component n-recognin 4
Gene symbol Ubr4
Other names/aliases Rbaf600
Species Rattus norvegicus
 Database cross references - Ubr4
ExoCarta ExoCarta_313658
Entrez Gene 313658
UniProt Q2TL32  
 Ubr4 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ubr4
Molecular Function
    ligase activity GO:0016874 IEA
    zinc ion binding GO:0008270 IEA
    calmodulin binding GO:0005516 IEA
    ubiquitin-protein transferase activity GO:0004842 ISS
Biological Process
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 ISS
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    cytoskeleton GO:0005856 IEA
    nucleus GO:0005634 IEA
    nucleoplasm GO:0005654 ISO
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
 Experiment description of studies that identified Ubr4 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 101
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ubr4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ubr4 is involved
No pathways found





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