Gene description for Ubr4
Gene name ubiquitin protein ligase E3 component n-recognin 4
Gene symbol Ubr4
Other names/aliases Rbaf600
Species Rattus norvegicus
 Database cross references - Ubr4
ExoCarta ExoCarta_313658
Vesiclepedia VP_313658
Entrez Gene 313658
UniProt Q2TL32  
 Ubr4 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ubr4
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    calmodulin binding GO:0005516 IEA
    calmodulin binding GO:0005516 ISO
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase activity GO:0061630 IEA
    ubiquitin protein ligase activity GO:0061630 ISO
    ubiquitin protein ligase activity GO:0061630 ISS
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    response to oxidative stress GO:0006979 IEA
    response to oxidative stress GO:0006979 ISO
    endosome organization GO:0007032 IEA
    endosome organization GO:0007032 ISO
    endosome organization GO:0007032 ISS
    positive regulation of autophagy GO:0010508 IEA
    positive regulation of autophagy GO:0010508 ISO
    positive regulation of autophagy GO:0010508 ISS
    protein ubiquitination GO:0016567 IEA
    protein catabolic process GO:0030163 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISS
    protein K27-linked ubiquitination GO:0044314 IEA
    protein K27-linked ubiquitination GO:0044314 ISO
    protein K27-linked ubiquitination GO:0044314 ISS
    negative regulation of fatty acid biosynthetic process GO:0045717 IEA
    negative regulation of fatty acid biosynthetic process GO:0045717 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    protein K48-linked ubiquitination GO:0070936 IEA
    protein K48-linked ubiquitination GO:0070936 ISO
    protein K48-linked ubiquitination GO:0070936 ISS
    protein K11-linked ubiquitination GO:0070979 IEA
    protein K11-linked ubiquitination GO:0070979 ISO
    protein K11-linked ubiquitination GO:0070979 ISS
    ubiquitin-dependent protein catabolic process via the N-end rule pathway GO:0071596 IEA
    ubiquitin-dependent protein catabolic process via the N-end rule pathway GO:0071596 ISO
    cytoplasm protein quality control by the ubiquitin-proteasome system GO:0071629 IEA
    cytoplasm protein quality control by the ubiquitin-proteasome system GO:0071629 ISO
    cytoplasm protein quality control by the ubiquitin-proteasome system GO:0071629 ISS
    cytoplasm protein quality control GO:0140455 ISO
    cytoplasm protein quality control GO:0140455 ISS
    negative regulation of HRI-mediated signaling GO:0141191 IEA
    negative regulation of HRI-mediated signaling GO:0141191 ISO
    negative regulation of HRI-mediated signaling GO:0141191 ISS
    protein branched polyubiquitination GO:0141198 IEA
    protein branched polyubiquitination GO:0141198 ISO
    protein branched polyubiquitination GO:0141198 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endosome GO:0005768 IEA
    endosome GO:0005768 ISO
    endosome GO:0005768 ISS
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Ubr4 in sEVs
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ubr4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rad23b  
Affinity Capture-MS Rattus norvegicus
2 MTNR1B  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Ubr4 is involved
No pathways found





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