Gene description for Ddi2
Gene name DNA-damage inducible protein 2
Gene symbol Ddi2
Other names/aliases RGD1311815
Species Rattus norvegicus
 Database cross references - Ddi2
ExoCarta ExoCarta_313668
Vesiclepedia VP_313668
Entrez Gene 313668
 Ddi2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Ddi2
Molecular Function
    aspartic-type endopeptidase activity GO:0004190 ISO
    identical protein binding GO:0042802 ISO
    ubiquitin binding GO:0043130 ISO
Biological Process
    proteasomal protein catabolic process GO:0010498 ISO
    protein processing GO:0016485 ISO
    regulation of protein stability GO:0031647 ISO
    cellular response to hydroxyurea GO:0072711 ISO
    regulation of DNA stability GO:0097752 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Ddi2 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ddi2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ddi2 is involved
No pathways found





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