Experiment description of studies that identified Ski in sEVs

1 Experiment ID 92 MISEV standards ✔ EM Biophysical techniques ✔ HSP70|GAPDH|CD9|CD151 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M." Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting RatRef-12 Microarray FACS Mass spectrometry [MALDI TOF]

2 Experiment ID 93 MISEV standards ✔ EM Biophysical techniques ✔ HSP70|GAPDH|CD9|CD151 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 20124479     Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M." Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS-Tspan8 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting

Protein-protein interactions for Ski

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Rnf111   Affinity Capture-Western Rattus norvegicus 2 Smurf2   Affinity Capture-Western Rattus norvegicus View the network

Pathways in which Ski is involved

No pathways found