Gene description for Myo1f
Gene name myosin IF
Gene symbol Myo1f
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Myo1f
ExoCarta ExoCarta_314654
Vesiclepedia VP_314654
Entrez Gene 314654
 Myo1f identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Myo1f
Molecular Function
    microfilament motor activity GO:0000146 IBA
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    actin filament binding GO:0051015 IBA
Biological Process
    neutrophil mediated immunity GO:0002446 ISO
    endocytosis GO:0006897 IBA
    actin filament organization GO:0007015 IBA
    negative regulation of cell adhesion GO:0007162 ISO
    response to bacterium GO:0009617 ISO
    regulation of actin cytoskeleton organization GO:0032956 ISO
    neutrophil degranulation GO:0043312 ISO
    regulation of innate immune response GO:0045088 ISO
    defense response to Gram-positive bacterium GO:0050830 ISO
    positive regulation of neutrophil migration GO:1902624 ISO
    neutrophil migration GO:1990266 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    microvillus GO:0005902 IBA
    actin cytoskeleton GO:0015629 IBA
    myosin complex GO:0016459 IEA
    cortical actin cytoskeleton GO:0030864 ISO
    filamentous actin GO:0031941 ISO
 Experiment description of studies that identified Myo1f in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Myo1f
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Myo1f is involved
No pathways found





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