Gene description for Nckap1l
Gene name NCK associated protein 1 like
Gene symbol Nckap1l
Other names/aliases Hem1
Species Rattus norvegicus
 Database cross references - Nckap1l
ExoCarta ExoCarta_315348
Vesiclepedia VP_315348
Entrez Gene 315348
 Nckap1l identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Nckap1l
Molecular Function
    GTPase activator activity GO:0005096 ISO
    GTPase activator activity GO:0005096 ISS
    protein kinase activator activity GO:0030295 ISO
    protein kinase activator activity GO:0030295 ISS
    protein-containing complex binding GO:0044877 ISO
    protein-containing complex binding GO:0044877 ISS
    TORC2 complex binding GO:1904841 ISO
Biological Process
    cell morphogenesis GO:0000902 IBA
    B cell homeostasis GO:0001782 ISO
    B cell homeostasis GO:0001782 ISS
    myeloid cell homeostasis GO:0002262 ISO
    myeloid cell homeostasis GO:0002262 ISS
    positive regulation of leukocyte migration GO:0002687 ISO
    chemotaxis GO:0006935 ISO
    chemotaxis GO:0006935 ISS
    response to xenobiotic stimulus GO:0009410 ISO
    response to xenobiotic stimulus GO:0009410 ISS
    cell migration GO:0016477 IBA
    maintenance of cell polarity GO:0030011 ISO
    maintenance of cell polarity GO:0030011 ISS
    cell projection assembly GO:0030031 IBA
    positive regulation of actin filament polymerization GO:0030838 ISO
    positive regulation of actin filament polymerization GO:0030838 ISS
    cortical actin cytoskeleton organization GO:0030866 IBA
    cortical actin cytoskeleton organization GO:0030866 ISO
    cortical actin cytoskeleton organization GO:0030866 ISS
    positive regulation of B cell proliferation GO:0030890 ISO
    positive regulation of B cell proliferation GO:0030890 ISS
    negative regulation of interleukin-17 production GO:0032700 ISO
    negative regulation of interleukin-17 production GO:0032700 ISS
    negative regulation of interleukin-6 production GO:0032715 ISO
    negative regulation of interleukin-6 production GO:0032715 ISS
    positive regulation of cell adhesion mediated by integrin GO:0033630 ISO
    positive regulation of cell adhesion mediated by integrin GO:0033630 ISS
    erythrocyte homeostasis GO:0034101 ISO
    erythrocyte homeostasis GO:0034101 ISS
    intracellular signal transduction GO:0035556 IEA
    intracellular signal transduction GO:0035556 ISO
    intracellular signal transduction GO:0035556 ISS
    positive regulation of T cell proliferation GO:0042102 ISO
    positive regulation of T cell proliferation GO:0042102 ISS
    T cell homeostasis GO:0043029 ISO
    T cell homeostasis GO:0043029 ISS
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of apoptotic process GO:0043066 ISS
    negative regulation of cytotoxic T cell degranulation GO:0043318 ISO
    positive regulation of CD4-positive, alpha-beta T cell differentiation GO:0043372 ISO
    positive regulation of CD4-positive, alpha-beta T cell differentiation GO:0043372 ISS
    positive regulation of CD8-positive, alpha-beta T cell differentiation GO:0043378 ISO
    positive regulation of CD8-positive, alpha-beta T cell differentiation GO:0043378 ISS
    positive regulation of B cell differentiation GO:0045579 ISO
    positive regulation of B cell differentiation GO:0045579 ISS
    positive regulation of gamma-delta T cell differentiation GO:0045588 ISO
    positive regulation of gamma-delta T cell differentiation GO:0045588 ISS
    positive regulation of lymphocyte differentiation GO:0045621 ISO
    positive regulation of lymphocyte differentiation GO:0045621 ISS
    positive regulation of erythrocyte differentiation GO:0045648 ISO
    positive regulation of erythrocyte differentiation GO:0045648 ISS
    neuron projection morphogenesis GO:0048812 IBA
    erythrocyte development GO:0048821 ISO
    B cell receptor signaling pathway GO:0050853 ISO
    B cell receptor signaling pathway GO:0050853 ISS
    positive regulation of phagocytosis, engulfment GO:0060100 ISO
    positive regulation of phagocytosis, engulfment GO:0060100 ISS
    protein-containing complex assembly GO:0065003 ISO
    protein-containing complex assembly GO:0065003 ISS
    actin polymerization-dependent cell motility GO:0070358 ISO
    actin polymerization-dependent cell motility GO:0070358 ISS
    positive regulation of neutrophil chemotaxis GO:0090023 ISO
    positive regulation of neutrophil chemotaxis GO:0090023 ISS
    positive regulation of neutrophil migration GO:1902624 ISO
    positive regulation of TORC2 signaling GO:1904515 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    SCAR complex GO:0031209 IBA
    SCAR complex GO:0031209 ISO
    SCAR complex GO:0031209 ISS
 Experiment description of studies that identified Nckap1l in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nckap1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nckap1l is involved
No pathways found





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