Gene description for Nckap1l
Gene name NCK associated protein 1 like
Gene symbol Nckap1l
Other names/aliases Hem1
Species Rattus norvegicus
 Database cross references - Nckap1l
ExoCarta ExoCarta_315348
Entrez Gene 315348
 Nckap1l identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Nckap1l
Molecular Function
    protein kinase activator activity GO:0030295 ISO
    GTPase activator activity GO:0005096 ISO
    protein complex binding GO:0032403 ISO
Biological Process
    chemotaxis GO:0006935 ISO
    negative regulation of interleukin-6 production GO:0032715 ISO
    positive regulation of gamma-delta T cell differentiation GO:0045588 ISO
    neutrophil chemotaxis GO:0030593 ISO
    B cell homeostasis GO:0001782 ISO
    erythrocyte development GO:0048821 ISO
    positive regulation of lymphocyte differentiation GO:0045621 ISO
    positive regulation of T cell proliferation GO:0042102 ISO
    negative regulation of myosin-light-chain-phosphatase activity GO:0035509 ISO
    negative regulation of interleukin-17 production GO:0032700 ISO
    positive regulation of CD8-positive, alpha-beta T cell differentiation GO:0043378 ISO
    positive regulation of erythrocyte differentiation GO:0045648 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of phosphorylation GO:0042327 ISO
    cortical actin cytoskeleton organization GO:0030866 ISO
    positive regulation of protein kinase activity GO:0045860 ISO
    B cell receptor signaling pathway GO:0050853 ISO
    maintenance of cell polarity GO:0030011 ISO
    positive regulation of neutrophil chemotaxis GO:0090023 ISO
    positive regulation of phagocytosis, engulfment GO:0060100 ISO
    positive regulation of CD4-positive, alpha-beta T cell differentiation GO:0043372 ISO
    positive regulation of actin filament polymerization GO:0030838 ISO
    protein complex assembly GO:0006461 ISO
    positive regulation of GTPase activity GO:0043547 ISO
    actin polymerization-dependent cell motility GO:0070358 ISO
    response to drug GO:0042493 ISO
    myeloid cell homeostasis GO:0002262 ISO
    positive regulation of B cell proliferation GO:0030890 ISO
    positive regulation of cell adhesion mediated by integrin GO:0033630 ISO
    T cell homeostasis GO:0043029 ISO
    positive regulation of B cell differentiation GO:0045579 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    extracellular exosome GO:0070062 ISO
    SCAR complex GO:0031209 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Nckap1l in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nckap1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nckap1l is involved
No pathways found





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