Gene description for Arih2
Gene name ariadne RBR E3 ubiquitin protein ligase 2
Gene symbol Arih2
Other names/aliases ARI2
TRIAD1
Species Rattus norvegicus
 Database cross references - Arih2
ExoCarta ExoCarta_316005
Vesiclepedia VP_316005
Entrez Gene 316005
 Arih2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Arih2
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
    zinc ion binding GO:0008270 IEA
    ubiquitin conjugating enzyme binding GO:0031624 IBA
    ubiquitin conjugating enzyme binding GO:0031624 ISO
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 ISO
Biological Process
    protein polyubiquitination GO:0000209 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    protein ubiquitination GO:0016567 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K48-linked ubiquitination GO:0070936 ISO
    hematopoietic stem cell proliferation GO:0071425 ISO
    positive regulation of protein targeting to mitochondrion GO:1903955 ISO
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    Cul5-RING ubiquitin ligase complex GO:0031466 ISO
 Experiment description of studies that identified Arih2 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Arih2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Arih2 is involved
No pathways found





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