Gene description for Atg4b
Gene name autophagy related 4B, cysteine peptidase
Gene symbol Atg4b
Other names/aliases Apg4b
Species Rattus norvegicus
 Database cross references - Atg4b
ExoCarta ExoCarta_316640
Vesiclepedia VP_316640
Entrez Gene 316640
 Atg4b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Atg4b
Molecular Function
    endopeptidase activity GO:0004175 ISO
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 IEA
    cysteine-type endopeptidase activity GO:0004197 ISO
    peptidase activity GO:0008233 ISO
    cysteine-type peptidase activity GO:0008234 IEA
    cysteine-type peptidase activity GO:0008234 ISO
    protein-phosphatidylethanolamide deconjugating activity GO:0019786 IBA
    protein-phosphatidylethanolamide deconjugating activity GO:0019786 IEA
    protein-phosphatidylethanolamide deconjugating activity GO:0019786 ISO
    scaffold protein binding GO:0097110 IEA
    scaffold protein binding GO:0097110 ISO
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 ISO
    mitophagy GO:0000423 IBA
    mitophagy GO:0000423 IEA
    mitophagy GO:0000423 ISO
    proteolysis GO:0006508 IEA
    proteolysis GO:0006508 ISO
    autophagy GO:0006914 IEA
    autophagy GO:0006914 ISO
    cellular response to starvation GO:0009267 ISO
    protein transport GO:0015031 IEA
    microautophagy GO:0016237 IEA
    microautophagy GO:0016237 ISO
    protein processing GO:0016485 IBA
    otolith mineralization completed early in development GO:0031173 ISO
    protein localization to phagophore assembly site GO:0034497 IEA
    protein localization to phagophore assembly site GO:0034497 ISO
    piecemeal microautophagy of the nucleus GO:0034727 IBA
    aggrephagy GO:0035973 IBA
    positive regulation of protein catabolic process GO:0045732 IMP
    protein delipidation GO:0051697 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 IEA
    autophagosome membrane GO:0000421 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IEA
    autophagosome GO:0005776 IEA
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IEA
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified Atg4b in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atg4b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atg4b is involved
No pathways found





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