Gene description for Atg4b
Gene name autophagy related 4B, cysteine peptidase
Gene symbol Atg4b
Other names/aliases Apg4b
Species Rattus norvegicus
 Database cross references - Atg4b
ExoCarta ExoCarta_316640
Entrez Gene 316640
 Atg4b identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Atg4b
Molecular Function
    molecular_function GO:0003674 ND
    endopeptidase activity GO:0004175 ISO
    cysteine-type endopeptidase activity GO:0004197 IBA
    peptidase activity GO:0008233 ISO
    cysteine-type peptidase activity GO:0008234 ISO
Biological Process
    protein delipidation GO:0051697 IBA
    mitochondrion degradation GO:0000422 IBA
    proteolysis GO:0006508 ISO
    protein processing GO:0016485 IBA
    cellular response to nitrogen starvation GO:0006995 IBA
    autophagosome assembly GO:0000045 ISO
    protein targeting to membrane GO:0006612 IBA
    C-terminal protein lipidation GO:0006501 IBA
    positive regulation of autophagy GO:0010508 ISO
    positive regulation of protein catabolic process GO:0045732 IMP
    autophagy GO:0006914 ISO
    nucleophagy GO:0044804 IBA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytoplasm GO:0005737 ISO
    cellular_component GO:0005575 ND
 Experiment description of studies that identified Atg4b in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atg4b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atg4b is involved
No pathways found





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