Gene description for Brcc3
Gene name BRCA1/BRCA2-containing complex, subunit 3
Gene symbol Brcc3
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Brcc3
ExoCarta ExoCarta_316794
Entrez Gene 316794
UniProt B2RYM5  
 Brcc3 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Brcc3
Molecular Function
    polyubiquitin binding GO:0031593 ISS
    metal ion binding GO:0046872 IEA
    ubiquitin-specific protease activity GO:0004843 ISS
    enzyme regulator activity GO:0030234 ISO
    metallopeptidase activity GO:0008237 ISS
Biological Process
    double-strand break repair GO:0006302 ISS
    positive regulation of DNA repair GO:0045739 ISS
    response to ionizing radiation GO:0010212 ISS
    response to X-ray GO:0010165 ISO
    protein K63-linked deubiquitination GO:0070536 ISS
    regulation of catalytic activity GO:0050790 ISO
    histone H2A K63-linked deubiquitination GO:0070537 ISS
Subcellular Localization
    nuclear ubiquitin ligase complex GO:0000152 ISO
    BRISC complex GO:0070552 ISS
    BRCA1-A complex GO:0070531 ISS
    ubiquitin ligase complex GO:0000151 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Brcc3 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Brcc3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Brcc3 is involved
No pathways found





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