Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.11-1.21 g/mL
Molecules identified in the study
Protein mRNA miRNA
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array
2
Experiment ID
263
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25242146
Organism
Mus musculus
Experiment description
Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors
Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.20 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
3
Experiment ID
264
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25242146
Organism
Mus musculus
Experiment description
Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors
Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool.
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