Gene description for Phf8
Gene name PHD finger protein 8
Gene symbol Phf8
Other names/aliases 9830141C09Rik
mKIAA1111
Species Mus musculus
 Database cross references - Phf8
ExoCarta ExoCarta_320595
Entrez Gene 320595
UniProt Q80TJ7  
 Phf8 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Phf8
Molecular Function
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors GO:0016706 ISO
    methylated histone binding GO:0035064 ISO
    iron ion binding GO:0005506 ISO
    histone demethylase activity GO:0032452 ISO
    histone demethylase activity (H3-K36 specific) GO:0051864 ISO
    histone demethylase activity (H3-K27 specific) GO:0071558 ISO
    chromatin binding GO:0003682 ISO
    histone demethylase activity (H3-K9 specific) GO:0032454 ISO
    histone demethylase activity (H4-K20 specific) GO:0035575 ISO
    zinc ion binding GO:0008270 ISO
Biological Process
    histone H3-K27 demethylation GO:0071557 ISO
    histone H4-K20 demethylation GO:0035574 ISO
    oxidation-reduction process GO:0055114 ISO
    G1/S transition of mitotic cell cycle GO:0000082 ISO
    histone H3-K36 demethylation GO:0070544 ISO
    histone H3-K9 demethylation GO:0033169 ISO
    positive regulation of transcription from RNA polymerase I promoter GO:0045943 ISO
    negative regulation of chromatin silencing at rDNA GO:0061188 ISO
    positive regulation of transcription, DNA-templated GO:0045893 ISO
Subcellular Localization
    nuclear membrane GO:0031965 ISO
    nucleolus GO:0005730 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Phf8 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Phf8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Phf8 is involved
PathwayEvidenceSource
Condensation of Prophase Chromosomes IEA Reactome
HDMs demethylate histones IEA Reactome





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