Gene description for rho-4
Gene name rhomboid-4
Gene symbol rho-4
Other names/aliases CG1697
DmelCG1697
Rho-4
Rho-related [10C6]
rho 4
rho4
Species Drosophila melanogaster
 Database cross references - rho-4
ExoCarta ExoCarta_32109
Vesiclepedia VP_32109
Entrez Gene 32109
 rho-4 identified in sEVs derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for rho-4
Molecular Function
    serine-type endopeptidase activity GO:0004252 IDA
    serine-type endopeptidase activity GO:0004252 IMP
    calcium ion binding GO:0005509 IDA
Biological Process
    protein processing GO:0016485 IDA
    epidermal growth factor receptor ligand maturation GO:0038004 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified rho-4 in sEVs
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for rho-4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Su(var)205  
Two-hybrid Drosophila melanogaster
2 LysD  
Two-hybrid Drosophila melanogaster
3 ninaE  
Two-hybrid Drosophila melanogaster
4 lark  
Two-hybrid Drosophila melanogaster
5 PlexA  
Two-hybrid Drosophila melanogaster
View the network image/svg+xml
 Pathways in which rho-4 is involved
No pathways found





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