Gene description for AP-2alpha
Gene name Adaptor Protein complex 2, alpha subunit
Gene symbol AP-2alpha
Other names/aliases AP-2
AP2
Alpha-Adaptin
CG31654
CG4260
D-Ada
D-alphaAda
DmelCG4260
MENE (2L)-A
MENE(2L)-A
ada
alpha
alpha-Ada
alpha-Adaptin
alpha-ada
alpha-adaptin
apl3
cg4260
dAP-2a
l(2)06694
l(2)SH0460
l(2)SH2 0460
Species Drosophila melanogaster
 Database cross references - AP-2alpha
ExoCarta ExoCarta_33211
Vesiclepedia VP_33211
Entrez Gene 33211
UniProt P91926  
 AP-2alpha identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for AP-2alpha
Molecular Function
    clathrin adaptor activity GO:0035615 IMP
Biological Process
    endocytosis GO:0006897 IMP
    protein localization GO:0008104 IMP
    asymmetric cell division GO:0008356 TAS
    positive regulation of autophagy GO:0010508 IMP
    protein transport GO:0015031 IDA
    regulation of endocytosis GO:0030100 IDA
    follicle cell of egg chamber development GO:0030707 IMP
    receptor internalization GO:0031623 IGI
    positive regulation of signal transduction by receptor internalization GO:0038010 IMP
    positive regulation of endocytosis GO:0045807 IMP
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle transport GO:0048489 TAS
    mitotic cleavage furrow ingression GO:1990386 IMP
Subcellular Localization
    plasma membrane GO:0005886 HDA
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 IDA
    AP-2 adaptor complex GO:0030122 IMP
 Experiment description of studies that identified AP-2alpha in exosomes
1
Experiment ID 202
MISEV standards
IEM
Biophysical techniques
TSG10|RAB35|FLOT2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AP-2alpha
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hexim  
Affinity Capture-MS Drosophila melanogaster
2 Afti  
Affinity Capture-Western Drosophila melanogaster
3 Reps  
Affinity Capture-MS Drosophila melanogaster
4 Nak  
Affinity Capture-MS Drosophila melanogaster
5 Leash  
Affinity Capture-MS Drosophila melanogaster
6 AP-1-2beta  
Affinity Capture-MS Drosophila melanogaster
7 Rlip  
Affinity Capture-MS Drosophila melanogaster
8 Vdup1  
Affinity Capture-MS Drosophila melanogaster
9 Ntf-2  
Affinity Capture-MS Drosophila melanogaster
10 CG42557  
Affinity Capture-MS Drosophila melanogaster
11 AP-2mu  
Affinity Capture-MS Drosophila melanogaster
12 lqf  
Affinity Capture-Western Drosophila melanogaster
13 AP-2sigma  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which AP-2alpha is involved
PathwayEvidenceSource
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
EPH-ephrin mediated repulsion of cells IEA Reactome
EPH-Ephrin signaling IEA Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Membrane Trafficking IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Neutrophil degranulation IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by WNT IEA Reactome
Trafficking of AMPA receptors IEA Reactome
Trafficking of GluR2-containing AMPA receptors IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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