ExoCarta: Gene summary

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Gene description for RpS2
Gene name	Ribosomal protein S2
Gene symbol	RpS2
Other names/aliases	CG5920 Dm01335 DmelCG5920 Rp S2 S2 anon-EST:Posey202 dS2 l(2)03848 l(2)k04603 sop
Species	Drosophila melanogaster
Database cross references - RpS2
ExoCarta	ExoCarta_34309
Entrez Gene	34309
UniProt	P31009
RpS2 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells	23035643

Gene ontology annotations for RpS2

Molecular Function
	RNA binding	GO:0003723	IEA
	structural constituent of ribosome	GO:0003735	NAS
Biological Process
	translation	GO:0006412	NAS
Subcellular Localization
	nucleolus	GO:0005730	IDA
	cytosolic small ribosomal subunit	GO:0022627	NAS
	nuclear chromosome	GO:0000228	IDA
	ribosome	GO:0005840	NAS
	cytoplasm	GO:0005737	IDA

Experiment description of studies that identified RpS2 in exosomes

Experiment ID

202

ISEV standards
✔ IEM	EV Biophysical techniques
✔ TSG10\|RAB35	EV Cytosolic markers
✔ FLOT2	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23035643

Organism

Drosophila melanogaster

Experiment description

Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.

Authors

Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP.

Journal name

Traffic

Publication year

2012

Sample

Drosophila S2 cells

Sample name

Normal-Drosophila S2 tub-Wg

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for RpS2

	Protein Interactor	ExoCarta ID	Identification method	PubMed	Species
No interactions are found.

Pathways in which RpS2 is involved

Pathway	Evidence	Source
Eukaryotic Translation Termination	IEA	Reactome
Formation of a pool of free 40S subunits	IEA	Reactome
Formation of the ternary complex, and subsequently, the 43S complex	IEA	Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit	IEA	Reactome
L13a-mediated translational silencing of Ceruloplasmin expression	IEA	Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)	IEA	Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)	IEA	Reactome
Peptide chain elongation	IEA	Reactome
Ribosomal scanning and start codon recognition	IEA	Reactome
SRP-dependent cotranslational protein targeting to membrane	IEA	Reactome
Translation initiation complex formation	IEA	Reactome

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