Gene description for Kif15
Gene name kinesin family member 15
Gene symbol Kif15
Other names/aliases Knsl7
Species Rattus norvegicus
 Database cross references - Kif15
ExoCarta ExoCarta_353302
Vesiclepedia VP_353302
Entrez Gene 353302
UniProt Q7TSP2  
 Kif15 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Kif15
Molecular Function
    microtubule motor activity GO:0003777 IBA
    microtubule motor activity GO:0003777 IEA
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IEA
    plus-end-directed microtubule motor activity GO:0008574 ISS
    ATP hydrolysis activity GO:0016887 IBA
Biological Process
    microtubule-based movement GO:0007018 IBA
    microtubule-based movement GO:0007018 IEA
    centrosome separation GO:0051299 ISS
    mitotic spindle assembly GO:0090307 ISS
Subcellular Localization
    spindle pole GO:0000922 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    spindle GO:0005819 IEA
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
 Experiment description of studies that identified Kif15 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Kif15
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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