Gene description for Gapdh1
Gene name Glyceraldehyde 3 phosphate dehydrogenase 1
Gene symbol Gapdh1
Other names/aliases BEST:GH12586
CG12055
DmelCG12055
GA3PDH
GADPH
GAP
GAPDH
GAPDH I
GAPDH-1
GAPDH1
GAPDHI
Gapd
Gapdh
Gapdh-1
Gapdh43E
gapdh
gapdh-1
gh12586
Species Drosophila melanogaster
 Database cross references - Gapdh1
ExoCarta ExoCarta_35728
Vesiclepedia VP_35728
Entrez Gene 35728
UniProt P07486  
 Gapdh1 identified in exosomes derived from the following tissue/cell type
Drosophila S2 cells 23035643    
 Gene ontology annotations for Gapdh1
Molecular Function
    glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity GO:0004365 IGI
Biological Process
    glycolytic process GO:0006096 IEP
    glyceraldehyde-3-phosphate metabolic process GO:0019682 IGI
Subcellular Localization
    Z disc GO:0030018 IDA
    M band GO:0031430 IDA
 Experiment description of studies that identified Gapdh1 in exosomes
1
Experiment ID 202
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG10|RAB35|FLOT2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23035643    
Organism Drosophila melanogaster
Experiment description Drosophila S2 cells secrete Wingless on exosome-like vesicles but the Wingless gradient forms independently of exosomes.
Authors "Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Borgne RL, Vincent JP."
Journal name Traffic
Publication year 2012
Sample Drosophila S2 cells
Sample name Normal-Drosophila S2 tub-Wg
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gapdh1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CTPsyn  
Proximity Label-MS Drosophila melanogaster
2 Mdh2  
Affinity Capture-MS Drosophila melanogaster
3 CG15153  
Two-hybrid Drosophila melanogaster
4 Parp  
Affinity Capture-MS Drosophila melanogaster
5 CG10887  
Affinity Capture-MS Drosophila melanogaster
6 cry  
Proximity Label-MS Drosophila melanogaster
7 betaTub85D  
Affinity Capture-MS Drosophila melanogaster
8 Hsc70-2  
Affinity Capture-MS Drosophila melanogaster
9 AnxB10  
Affinity Capture-MS Drosophila melanogaster
10 Gapdh2  
Affinity Capture-MS Drosophila melanogaster
11 CG8353  
Affinity Capture-MS Drosophila melanogaster
12 Tom20  
Proximity Label-MS Drosophila melanogaster
13 ebo  
Two-hybrid Drosophila melanogaster
14 NC2alpha  
Two-hybrid Drosophila melanogaster
15 CG13074  
Two-hybrid Drosophila melanogaster
16 CG10638  
Affinity Capture-MS Drosophila melanogaster
17 regucalcin  
Affinity Capture-MS Drosophila melanogaster
18 zuc  
Proximity Label-MS Drosophila melanogaster
19 bs  
Affinity Capture-MS Drosophila melanogaster
20 RpS15Ab  
Affinity Capture-MS Drosophila melanogaster
View the network image/svg+xml
 Pathways in which Gapdh1 is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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