Gene description for Rnasel
Gene name ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
Gene symbol Rnasel
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rnasel
ExoCarta ExoCarta_359726
Entrez Gene 359726
 Rnasel identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Rnasel
Molecular Function
    ATP binding GO:0005524 IEA
    RNA binding GO:0003723 ISO
    rRNA binding GO:0019843 IDA
    ribonucleoprotein complex binding GO:0043021 ISO
    ribonuclease activity GO:0004540 IDA
    protein kinase activity GO:0004672 IEA
Biological Process
    RNA phosphodiester bond hydrolysis GO:0090501 IEA
    rRNA processing GO:0006364 IDA
    protein phosphorylation GO:0006468 IEA
    defense response to virus GO:0051607 ISO
    mRNA processing GO:0006397 IEA
    negative regulation of viral genome replication GO:0045071 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    positive regulation of glucose import in response to insulin stimulus GO:2001275 ISO
    regulation of mRNA stability GO:0043488 ISO
    fat cell differentiation GO:0045444 ISO
Subcellular Localization
    nuclear matrix GO:0016363 IDA
 Experiment description of studies that identified Rnasel in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Rnasel
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rnasel is involved
No pathways found





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