Gene description for Nt5c3b
Gene name 5'-nucleotidase, cytosolic IIIB
Gene symbol Nt5c3b
Other names/aliases Nt5c3l
Species Rattus norvegicus
 Database cross references - Nt5c3b
ExoCarta ExoCarta_360629
Vesiclepedia VP_360629
Entrez Gene 360629
UniProt Q6AYP7  
 Nt5c3b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Nt5c3b
Molecular Function
    nucleotide binding GO:0000166 IEA
    magnesium ion binding GO:0000287 IEA
    5'-nucleotidase activity GO:0008253 IBA
    5'-nucleotidase activity GO:0008253 IEA
Biological Process
    nucleotide metabolic process GO:0009117 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Nt5c3b in sEVs
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nt5c3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nt5c3b is involved
PathwayEvidenceSource
Deadenylation-dependent mRNA decay IEA Reactome
Metabolism of RNA IEA Reactome
mRNA decay by 3' to 5' exoribonuclease IEA Reactome





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