ExoCarta: Gene summary

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Gene description for Cops4
Gene name	COP9 signalosome subunit 4
Gene symbol	Cops4
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Cops4
ExoCarta	ExoCarta_360915
Vesiclepedia	VP_360915
Entrez Gene	360915
UniProt	Q68FS2
Cops4 identified in sEVs derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Cops4

Molecular Function
	protein binding	GO:0005515	IPI
Biological Process
	protein deneddylation	GO:0000338	IEA
	protein deneddylation	GO:0000338	ISO
	protein deneddylation	GO:0000338	ISS
Subcellular Localization
	nucleus	GO:0005634	ISO
	cytoplasm	GO:0005737	ISO
	synaptic vesicle	GO:0008021	IDA
	synaptic vesicle	GO:0008021	IEA
	synaptic vesicle	GO:0008021	ISO
	COP9 signalosome	GO:0008180	IBA
	COP9 signalosome	GO:0008180	IEA
	COP9 signalosome	GO:0008180	ISO
	nuclear speck	GO:0016607	IEA
	nuclear speck	GO:0016607	ISO
	cell junction	GO:0030054	ISO

Experiment description of studies that identified Cops4 in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP90\|HSC70\|MHCI	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting