Gene description for Wdr1
Gene name WD repeat domain 1
Gene symbol Wdr1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Wdr1
ExoCarta ExoCarta_360950
Vesiclepedia VP_360950
Entrez Gene 360950
UniProt Q5RKI0  
 Wdr1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Wdr1
Molecular Function
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
    actin filament binding GO:0051015 ISS
Biological Process
    neutrophil mediated immunity GO:0002446 ISO
    regulation of cell shape GO:0008360 ISO
    actin cytoskeleton organization GO:0030036 ISO
    actin filament depolymerization GO:0030042 IBA
    actin filament fragmentation GO:0030043 ISO
    platelet formation GO:0030220 ISO
    regulation of actin filament depolymerization GO:0030834 ISO
    positive regulation of actin filament depolymerization GO:0030836 ISO
    cortical cytoskeleton organization GO:0030865 ISO
    locomotion GO:0040011 IBA
    establishment of planar polarity of follicular epithelium GO:0042247 ISO
    apical junction assembly GO:0043297 ISO
    maintenance of epithelial cell apical/basal polarity GO:0045199 ISO
    sarcomere organization GO:0045214 IBA
    sarcomere organization GO:0045214 ISO
    regulation of oligodendrocyte differentiation GO:0048713 IDA
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 ISO
    neutrophil migration GO:1990266 ISO
Subcellular Localization
    podosome GO:0002102 IEA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 ISO
    actin cytoskeleton GO:0015629 IDA
    cell junction GO:0030054 ISO
    cortical actin cytoskeleton GO:0030864 IBA
    cell projection GO:0042995 IEA
    synapse GO:0045202 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Wdr1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Wdr1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sumo3  
Affinity Capture-MS Rattus norvegicus
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